| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
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| Autores: |
Bombonato,Juliana Rodrigues
Bonatelli,Isabel Aparecida Silva
Silva,Gislaine Angélica Rodrigues
Moraes,Evandro Marsola
Zappi,Daniela Cristina
Taylor,Nigel P.
Franco,Fernando Faria
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| Data: |
2019-03-01
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| Ano: |
2019
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| Palavras-chave: |
Cactaceae
Cereus
Cross-genera
SSR markers
Transferability
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| Resumo: |
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/1678-4685-gmb-2017-0293
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.42 n.1 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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